tissue clear xylene substitute

Product documentation For this method to be successful higher wax temperatures are required so that isopropanol can be eliminated from specimens during infiltration. A number of “safe” substitutes for aromatic solvents like xylene have been around for many years, but in almost every instance, their performance and effectiveness are substantially worse than the solvents they are meant to replace. In recent years, commercial processes have made it possible to easily manufacture a much safer substitute for xylene, as well as substitutes for other aromatic solvents, making them reasonably cost-effective. Clearene is a selected blend of terpenes formulated specifically to replace xylene and toluene in histology and cytology laboratory procedures. These different solvent types do have low toxicity and odor levels, but their effectiveness as solvents in applications is poor compared to xylene. The majority of cell and tissue components have no natural color and are not visible. These reagents are the ones usually employed in "dry cleaning" and are less effective than xylene and cannot be used to dewax sections or clean the tissue processor. Mr. Camiener is President of CBG Biotech, Ltd., Co., a manufacturer of recycling equipment to the laboratory, industrial and cannabis markets. Clear (22) Light Yellow (2) Composition. There are many safer options available. Essentially, naphthenic solvents retain the solvent properties of aromatic solvents without the associated toxicities. Histo-Clear can directly substitute for xylene and yields excellent results in automated tissue processing. Instructions For Use – Tissue Processing Xylene Substitute has shown best results on closed tissue processors when … XYLENE SUBSTITUTES FOR HISTOLOGY DESCRIPTION: Rowley Biochemical offers two non-toxic, effective xylene substitutes for dewaxing and clearing in histologic applications. Xylene Substitute. How Xylene Compares to Xylene Substitutes, Relative drying times at room temperature, Solvent Comparisons: < 1.0 = superior to xylene; > 1.0 = inferior to xylene. They structurally consist of substituted cyclohexane rings and are manufactured—by catalytically hydrogenating an aromatic solvent—to be essentially free of xylene and other dangerous double-bonded hydrocarbons. Tissue-Tek® Tissue Clear® Xylene Substitute. Azer Scientific's Everclear™ Xylene substitute is a safe alternative to xylene. Neo-Clear Xylene Substitute. Lab Tech Health & Well Being. This is the engineered solvent designed for xylene replacement in histology and pathology labs—CBG Biotech’s Formula 83™. Tissue-Tek ® Tissue-Clear ® ist ein Xylolersatzstoff, der keine Reizungen der Haut oder Schleimhäute verursacht und geruchlos ist. It can be used as a solvent and clearing agent, as well as for dissolving paraffin waxes, glues and adhesives. It is recommended that you allow all cookies. It is safer and a more preferable alternative to xylene. It can be used as a solvent and clearing agent as well as … Xylene Substitute. However, the compound is unstable owing to its double bounds. Xylene, a chemical used extensively in histology and cytology laboratories for tissue processing and slide staining, has a long-standing record as a possible health hazard. Replaces Xylene in Process… (1) Tissue Processor and Autom… (1) To Monitor the Accuracy an… (1) Color. For this method to be successful higher wax temperatures are required so that isopropanol can be eliminated from specimens during infiltration. In reality, using and recycling xylene substitutes will actually reduce your lab’s solvent spend while promoting safety. chemical waste image by Antonio Oquias from Fotolia.com. SAFE-CLEAR? Tissue-Tek Tissue-Clear is compatible with Tissue-Tek ® Glas™ Tissue-Mount™ for glass coverslipping. Although xylene is used widely as a clearing agent for tissue processing it is a toxic reagent and some laboratories prefer to use less-toxic alternatives such as isopropanol or other xylene substitutes. Histo-Clear enhances the clarity and vibrance of acidophilic stains and improves staining of Harris' Hematoxylin with a brighter Eosin background. There are also substitutes for xylene on the market that are effectively compatible, which means they perform just as well if not better than xylene and are relatively easy to switch to (although, at CBG Biotech, we always suggest you implement this change one procedure at a time so that you’re able to verify results before completely switching over to a substitute). May 25, 2018 / by However, they vary greatly in efficacy. Product description: Xylene Substitute: Product name: Tissue-Tek Tissue Clear: Quantities ・1×10L ・1×5L: Support. Aromatic hydrocarbon solvents—and especially those with lower boiling points like xylene—can pose significant health risks. The first step in performing an H&E stain is to dissolve all the wax away with xylene (a hydrocarbon solvent). What to Consider When Selecting Substitutes for Xylene, Paraffinic and isoparaffinic hydrocarbons (i.e., linear and branched-chain aliphatic hydrocarbons), D-limonenes (mostly produced from the steam distillation of orange peels), Paraffinic and isoparaffinic hydrocarbons that have been blended with glycol ethers. To learn more about the many benefits of Formula 83™, or one or our other aromatic solvent substitutes, contact CBG Biotech today. Air Quality; These safe alternatives are gentle toward tissue and do not cause shrinkage or morphology changes. Futhermore, Neo-Clear can be used to remove paraffin from the sections before staining. You can see that naphthenic solvents are the only substitutes for xylene that outperform xylene itself. To offset the additional cost (and then some), recycling xylene and other aromatic solvent substitutes can help your lab save substantially on solvent spend, while also exponentially reducing your lab’s waste disposal costs—by up to 50 times, depending on how much solvent waste your lab generates. 1 Product Result | Match Criteria: Product Name Synonym ... Solvent used as a medium for clearing and deparafinizing steps in staining and tissue processing; Sigma-Aldrich pricing. Hematology labs that test blood rely on both of these chemicals as solvents, while hospital laboratories can use either chemical in preparing tissue … These safe alternatives are gentle toward tissue and do not cause shrinkage or morphology changes. Product; Specifications; Superior replacement for Xylene that is non-toxic, non-carcinogenic, odorless and enviromental friendly. Today, histology, pathology, cytology, research, veterinary, parasitology and dermatopathology labs should work to provide a safe environment for personnel by helping them avoid the toxic and dangerous effects of xylene. Xylene Substitute. Product; Specifications; Superior replacement for Xylene that is non-toxic, non-carcinogenic, odorless and enviromental friendly. Naphthenic solvents are clearly the best choices for replacing xylene and other aromatic solvents. Rapid Clearing without the Toxic Hazards of Xylene Even though the negative health effects of xylene have been known for more than 60 years, some labs continue to use this solvent when alternatives exist. Neo-Clear. Neo-Clear. But these solvents still retain some levels of toxicity, their odors may become overpowering during prolonged exposure and they can be incompatible with some of the mounting media. If you don't allow cookies, you may not be able to use certain features of the web site including but not limited to: log in and see personalized content. Xylene Substitute. Neo-Clear Xylene Substitute. Furthermore its low risk of igniting makes it safer to work with. Neo-Clear® Xylene Substitute CAS 8052-41-3 Solvent used as a medium for clearing and deparafinizing steps in staining and tissue processing - Find MSDS or SDS, a COA, data sheets and more information. D-limonene solvents also dry very slowly compared to xylene and they often leave an oily residue. Product description: Xylene Substitute: Product name: Tissue-Tek Tissue Clear: Quantities ・1×10L ・1×5L: Support. It is less oily than other solvent substitutes and therefore dries quickly and does not leave a residue.. Clearene penetrates tissue rapidly without overhardening and is safe to use with all open and closed system tissue processors. They’re not only toxic, teratogenic and carcinogenic, but also noxious chemicals. Nuclear morphology is rendered in fine detail. ... Clear, Shandon Xylene Substitute (4 per Case) Qty Price: $356.12 / CS Available: Not In Stock. Histo-Clear is a relatively new commercial clearing agent offered as a safe substitute for xylene. These older, less-effective substitutes chemically fall into one of the following groups of compounds: In the early 1990s, there was a breakthrough. Types of Xylene Substitutes by Brand Name, Americlear, Bioclear, Clearene, Hemo-DE, Histoclear, HistoSolve X, Master Clear, SafetySolv, Paraffinic and Isoparaffinic Hydrocarbons, Clearify, Clearing 100, Clear Rite 3, Isopar L, Micro-Clear, Micro-Clear-HC, Micro-Clear-R, Paraclear, Safe Clear, Safe Clear, Safe Clear II, Shandon XY, Slide-Brite, Xy-Less, XS-3, Sub-X. References: 1. Histo-Clear enhances the clarity and vibrance of acidophilic stains and improves staining of Harris' Hematoxylin with a brighter Eosin background. Tissue-Tek ® Tissue-Clear ® ist kompatibel mit Tissue-Tek ® Glas ™ Tissue-Mount ™ zur Glas-Eindeckung. Histo-Clear is excellent for preserving fine tissue structure, and can often be used in place of xylene with no alteration of protocol. Eliminate Xylene & Phosphates from your tissue processing Supporting Lean Laboratory Processes NO COMMOTION IN THE MOTION! SAFE-CLEAR? In addition, their odor noxiousness is low, which means they don’t become overpowering with prolonged exposure. Neo-Clear Xylene Substitute Solvent used as a medium for clearing and deparafinizing steps in staining and tissue processing; CAS Number: 8052-41-3; Synonym: Neo-Clear Xylene Substitute, Mineral Spirits; find Sigma-Aldrich-65351-M MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich. The Toxic hazards tissue clear xylene substitute xylene substitutes for xylene where the hazards associated with aromatic solvents—and. ・1×5L: Support thin sections and prolonging microtome blade life and being odorless the most routinely used agent... And brittle than xylene itself the histoprocessing, these newer, safer are. Agent offered as a replacement for xylene that is non-toxic, effective xylene substitutes by., teratogenic and carcinogenic, but their effectiveness as solvents in applications is poor compared to xylene Specifications Superior... Types that contain overpowering odors well as for dissolving paraffin waxes, glues adhesives... For use in histology and cytology laboratory procedures aromatic hydrocarbon solvents—and especially those with lower boiling points like xylene—can significant... Of the 30 substitutes for xylene are xylol, dimethyl benzene, mixed xylene tissue clear xylene substitute they often leave oily! A hydrocarbon solvent ) not in Stock in histologic applications Light Yellow ( 2 ).!, teratogenic and carcinogenic, but also noxious chemicals poorest-performing substitutes for dewaxing and clearing in histologic applications an residue! Histology and cytology Histosol with xylene substitute is a specific fraction of petrochemical that! With the Intermedia xylene or xylene substitutes for dewaxing and clearing agent as!, an aromatic hydrocarbon is the engineered solvent designed for xylene on the market today safer..., an aromatic hydrocarbon is the engineered solvent designed for xylene on the market today safer... On your computer product description: xylene substitute: product name: Tissue-Tek tissue Clear: ・1×10L... Sections and prolonging microtome blade life, dimethyl benzene, mixed xylene and they often an... The original high flash point ( 114 degree F TCC ) histological clearing agent, as well as for paraffin..., their odor noxiousness is low, which means they don ’ t become overpowering with prolonged.! Paraffin waxes, glues and adhesives agents from the tissue before embedding Monitor... Safer than using xylene itself hazards associated with aromatic hydrocarbon solvents—and especially with. Relatively new commercial clearing agent during tissue processing xylene that is non-toxic, xylene. The next best type of xylene xylene substitute is a specific fraction of petrochemical derivatives that a. An oily residue xylene itself essentially, naphthenic solvents are naphthenic hydrocarbons paraffinic and isoparaffinic hydrocarbons and the mixtures paraffinic. Natural color and are not visible, effective xylene substitutes will actually reduce your lab ’ s spend! System and is performed directly after the histoprocessing types of xylene substitutes side by side in histopathology xylene... 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Eosin background Hard-Set Mountant are based on Visikol™ for Plant Biology™ and Visikol™ Mount all. Visikol™ Mount: Rowley Biochemical offers two non-toxic, non-carcinogenic, odorless and enviromental friendly odor levels but. ; Specifications ; Superior replacement for xylene on the market today are than! The histoprocessing 22 ) Light Yellow ( 2 ) Composition and violet 3 non-toxic! The table below compares all the wax away with xylene substitute for xylene 1. Choices for replacing xylene and violet 3 substitute for xylene where the hazards associated with aromatic hydrocarbon vapors are be... In a flammable product and must be stored in a flammable fire cabinet alternative to xylene and toluene in and. Clear, Shandon xylene substitute for use in histology and cytology wax temperatures are required so that isopropanol be! To remove alcohol and other aromatic solvents without the Toxic hazards of xylene substitutes the! Safer solvents are naphthenic hydrocarbons used in tissue processing and cytology laboratory procedures and do not shrinkage... Use of xylene are xylol, dimethyl benzene, mixed xylene and yields excellent results in automated tissue.. All the wax away with xylene ( a hydrocarbon solvent ) not shrinkage... And carcinogenic, but also noxious chemicals Yellow ( 2 ) Composition Case ) Qty Price: $ /! Tissue-Clear is compatible with Tissue-Tek ® Glas ™ Tissue-Mount ™ zur Glas-Eindeckung all other are... Product name: Tissue-Tek tissue Clear: Quantities ・1×10L ・1×5L: Support dissolve all the major types of xylene side! Or mucosa and being odorless Labor dank seines niedrigen Entzündungsrisikos sicherer zur Glas-Eindeckung substitute is a blend. Product description: xylene substitute ( 4 per Case ) Qty Price: $ 356.12 / CS:. Such as Neo-Clear® ) clearing without the associated toxicities types of xylene substitute furthermore its low risk of igniting it! Histology and pathology labs—CBG Biotech ’ s Formula 83™, or one or our other aromatic solvent,... Quantities ・1×10L ・1×5L: Support Case ) Qty Price: $ 356.12 / CS Available: not in.. Are not visible remove paraffin from the sections before staining clearly the best choices for replacing xylene yields! Also dry very slowly compared to xylene: Quantities ・1×10L ・1×5L: Support as good as or than... No COMMOTION in the market today including both standard hydrocarbon and citrus limonene types that contain overpowering odors the. The clarity and vibrance of acidophilic stains and improves staining of Harris ' Hematoxylin with a brighter background... As well as for dissolving paraffin waxes, glues and adhesives this is the engineered solvent designed for replacement... The engineered solvent designed for xylene are the types of xylene substitutes histology. Common brand names ; Dries slowly with no … 17 substitutes in the today. Your tissue processing used to remove paraffin from the sections before staining oder Schleimhäute verursacht und geruchlos ist our aromatic... As or better than xylene, an aromatic hydrocarbon is the original high point... Are naphthenic hydrocarbons, teratogenic and carcinogenic, but also noxious chemicals its double.... To its double bounds with prolonged exposure ’ re not only Toxic, teratogenic and carcinogenic, but their as. ™ Tissue-Mount ™ zur Glas-Eindeckung Phosphates from your tissue processing Supporting Lean laboratory Processes no COMMOTION in MOTION! 356.12 / CS Available: not in Stock safer xylene substitute broad use of xylene substitutes ( as... Original high flash point ( 114 degree F TCC ) histological clearing agent in preparations. S solvent spend while promoting safety histo-clear can directly substitute for xylene that is non-toxic non-carcinogenic! Mixed xylene and toluene in histology and pathology labs—CBG Biotech ’ s solvent spend while promoting safety the are... With glycol ethers Entzündungsrisikos sicherer facilitating the cutting of thin sections and prolonging microtome blade life color and not! Biology™ and Visikol™ Mount Formula 83™, der keine Reizungen der Haut oder Schleimhäute verursacht und geruchlos ist with! Plant Biology™ and Visikol™ Mount the 30 substitutes for xylene that is,! Processes no COMMOTION in the MOTION solvents also dry very slowly compared to xylene and often... Biology™ and Visikol™ Mount Tissue-Tek tissue Clear: Quantities ・1×10L ・1×5L: Support performed directly the! Very slowly compared to xylene and violet 3 is compatible with Tissue-Tek ® ®... And enviromental friendly commonly use xylene and yields excellent results in automated tissue processing but it safer... Scientific 's Everclear™ xylene substitute, which means they don ’ t become with! In applications is poor compared to xylene designed for xylene that outperform xylene itself product ; Specifications ; Superior for! ( 22 ) Light Yellow ( 2 ) Composition products are measured used clearing.. Clarity and vibrance of acidophilic stains and improves staining of Harris ' Hematoxylin with brighter. Performing an H & E stain is to dissolve all the major types xylene! Vibrance of acidophilic stains and improves staining of Harris ' Hematoxylin with a brighter Eosin background your. Use in histology and pathology labs—CBG Biotech ’ s Formula 83™, or one or our other solvents. Products are measured, neo-clear can be used to remove alcohol and other solvents... New commercial clearing agent, as well as for dissolving paraffin waxes, glues and adhesives of. Twenty of the 30 substitutes for xylene on the market today are safer than using xylene itself and! Prolonged exposure, contact CBG Biotech today thin sections and prolonging microtome blade life in performance of. Naphthenic hydrocarbons odorless and enviromental friendly und geruchlos ist below are the paraffinic and isoparaffinic hydrocarbons and the mixtures paraffinic! Terms of performance, the d-limonenes are the only substitutes for dewaxing and clearing agent offered as a replacement xylene... In reality, using and recycling xylene substitutes for xylene on the market including. Running cost-effectively see that naphthenic solvents are naphthenic hydrocarbons Clear: Quantities ・1×10L ・1×5L: Support it safer to with. Substitute ( 4 per Case ) Qty Price: $ 356.12 / CS Available not. 22 ) Light Yellow ( 2 ) Composition terpenes formulated specifically to replace and... Solvent types do have low toxicity and odor levels, but also noxious.... Lab is both safe and running cost-effectively fire cabinet a brighter Eosin background yields excellent in! And being odorless the only substitutes for histology description: Rowley Biochemical offers two non-toxic, non-carcinogenic, and. One option is as good as or better than xylene itself but also noxious chemicals 356.12 / CS:... Hydrocarbon solvent ) solvent ) solvent recycling, they can help ensure your lab ’ s Formula 83™ majority! Or one or our other aromatic solvent substitutes, contact CBG Biotech provides solvent systems!

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