conjunctivitis in mice

Mice. Ten days after immunization, allergic conjuncti - vitis was induced by … A probable link between plasminogen and ligneous conjunctivitis has been indicated by the recent diagnoses of plasminogen deficiency in five patients suffering from ligneous conjunctivitis. conjunctival lesions, conjunctivitis, ligneous, mice, plasminogen, fibrin, epithelium, conjunctiva LIGNEOUS CONJUNCTIVITIS is a rare form of chronic membranous conjunctivitis of unknown origin. Viral conjunctivitis is the most common infectious conjunctivitis — the majority of cases are caused by adenoviruses. Pathogens. Experimental allergic conjunctivitis was induced by a modified version of a previously described protocol (Fig. 24 hours after the The pollinosis caused by pollen of Japanese cedar The Pathogenesis of Staphylococcus aureus Eye Infections. Methods: EC was induced in Balb/c mice by active immunisation with short ragweed pollen (RW) in alum. Viral conjunctivitis is the most common infectious conjunctivitis — the majority of cases are caused by adenoviruses. Twenty minutes after the last challenge, the clinical appearance was evaluated. Briefly, mice were previously immunized with SRW pollen combined with Inject Alum Adjuvant (50 μg in 5 mg) by foot-pad injection. Each value represents the mean±S.E.M. 2020 Oct 7;13(10):295. doi: 10.3390/ph13100295. Ligneous conjunctivitis is a rare form of chronic pseudomembranous conjunctivitis that is associated with systemic membranous pathological changes. Supported by a Career Development Award from Research to Prevent Blindness, New York, NY (A.H.K.) are involved in the development of experimental allergic conjunctivitis (EC) in mice. allergic conjunctivitis in mice through alterations in inflamma-tion, NGF and VEGF. Int J Pharm. Efficacy of oral immunotherapy with a rice-based edible vaccine containing hypoallergenic Japanese cedar pollen allergens for treatment of established allergic conjunctivitis in mice. Muc1 antibody staining of wild-type sections revealed the presence of Muc1 on conjunctival goblet and non-goblet cells and on the epithelium of the Harderian gland. The mouse model of allergic conjunctivitis was induced by SRW pollen. The incidence of lesion development in mice increases with age and is strongly influenced by genetic background. Sigma-Aldrich offers abstracts and full-text articles by [A F Drew, A H Kaufman, K W Kombrinck, M J Danton, C C Daugherty, J L Degen, T H Bugge]. Invest Ophthalmol Vis Sci. Contrary to various popular beliefs, conjunctivitis can have many causes besides bacterial infections. 1A). Serial sections stained with cytokeratin 14 antibody confirmed the epithelial nature of cells expressing the Muc1 protein. Patients with ligneous conjunctivitis present with pseudomembranous “woodlike” lesions of the conjunctiva which result in scarring and impaired vision. Male mice spent more time lying down in the smaller cage whereas the female mice showed an increase in grooming and sitting behaviors. Muc1 mRNA and protein are present in murine conjunctival and Harderian gland epithelia. Purpose: tal conjunctivitis (EC) in mice and to evaluate whether or not this model is suitable for the evaluation of eye drops conatin-ing anti-allergic agents. Treatment with olopatadine and naphazoline hydrochloride reduces allergic conjunctivitis in mice through alterations in inflammation, NGF and VEGF. Experimental allergic conjunctivitis was induced by a modified version of a previously described protocol (Fig. Histamine is the most important mediator in allergic conjunctivitis. Clipboard, Search History, and several other advanced features are temporarily unavailable. Influence of miR155 on allergic conjunctivitis in mice via regulation of NF-κB signal pathway. 2000 Dec;41(13):4080-4. Bacterial analysis of conjunctival swabs and whole eye preparations demonstrated the presence of coagulase-negative Staphylococcus, Streptococcus type alpha, and Corynebacterium group G2. One hour after the model was established, conjunctiva of mice … 2018 Jan 10;7(1):9. doi: 10.3390/pathogens7010009. Methods: One hour after the model was established, conjunctiva of mice were removed for further analysis. Copyright © 1998 American Society of Hematology. A … Mice were actively immunized with ovalbumin, conjunctivitis was induced by topical instillation of ovalbumin. Normal rat IgG was used for control injections. Prevention of allergic conjunctivitis in mice by a rice-based edible vaccine containing modified Japanese cedar pollen allergens Ken Fukuda,1 Waka Ishida,1 Yosuke Harada,1 Yuhya Wakasa,2 Hidenori Takagi,2 Fumio Takaiwa,2 Atsuki Fukushima1 1Department of … Zahorsky-Reeves et al. Invest Ophthalmol Vis Sci. In temperate climates, adenoviruses are most active during spring, early summer and mid-winter. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. Ligneous Conjunctivitis in Plasminogen-Deficient Mice. The aim of this study was to evaluate the effects of TQ on ovalbumin (OVA)-induced allergic conjunctivitis (AC) in Balb/c. HHS from the American Heart Association (93002570). Ligneous conjunctivitis is a rare form of chronic pseudomembranous conjunctivitis that is associated with systemic membranous pathological changes. Cells. Animals lacking Muc1 mRNA and protein are predisposed to developing eye inflammation when compared to wild-type animals with an intact Muc1 gene. Molecular approaches confirmed the presence of Muc1 mRNA and protein in the conjunctival tissue of wild-type mice and identified the bacterial species in Muc1 null symptomatic mice. An allergic conjunctivitis mouse model was established using histamine or an antigen (ovalbumin), following which mice were treated with 1% olopatadine solution and/or 0.2 mg/ml of naphazoline hydrochloride. U01 HD29963/HD/NICHD NIH HHS/United States, NCI CPTC Antibody Characterization Program. 2017 Nov 29;6(12):110. doi: 10.3390/jcm6120110. conjunctivitis in mice by eliciting immune tolerance. Histamine is the most important mediator in allergic conjunctivitis. Furthermore, it suggests the possibility that some epitopes recognized by Fabs could be used as a tool to regulate allergic conjunctivitis. NIH BALB/c mice actively sensitized with JCP were repeatedly challenged by topical administration of JCP eye drops. This study provides direct evidence that plasminogen deficiency is one cause of ligneous conjunctivitis and suggests that plasminogen-deficient mice may be an excellent model for the development of therapeutic strategies for the treatment of this debilitating disease. 10 days later (day 10), the mice were challenged with eye drops containing RW. Infective conjunctivitis is inflammation of the conjunctiva due to viral, bacterial or parasitic infection. Contrary to various popular beliefs, conjunctivitis can have many causes besides bacterial infections. Mengtian Bai 1-5, Yan Li 1-5, Yun Li 1-6, Zhulin Hu 1-5. for five mice. 7– 9 Immunohistochemical analyses of conjunctivas from rats 8 and mice 9 developing EC demonstrated … Conjunctivitis was induced in C57BL/6 mice by topical instillation of compound 48/80 (C48/80). (1/94) PURPOSE: In contrast to wild-type mice, genetically engineered Mucin1 (Muc1) null animals display a marked propensity for development of blepharitis and conjunctivitis. We have previously shown that high and low doses of oral antigen induced oral tolerance according to different mechanisms for OVA-induced allergic conjunctivitis in mice.2 Whereas high-dose treatment suppressed allergic conjunctivitis-related cytokine production and serum Ig values, T reg cells played a role in the induction of oral tolerance by low-dose treatment. This novel prophylactic approach is potentially safe and effective for allergen-specific oral immunotherapy in allergic conjunctivitis. Here, we investigate the efficacy and the mechanism of a sublingually administered Dermatophagoides farinae (Der f) vaccine in a murine AC model. Mechanistic analyses using rats and mice have confirmed that Ag specific CD4+ T cells play a crucial part in the development of experimental AC (experimental immune mediated blepharoconjunctivitis, EC) in terms of eosinophil infiltration into the conjunctiva. Detection of sialomucin complex (MUC4) in human ocular surface epithelium and tear fluid. To assess the anti-allergic mechanisms of tofacitinib, RBL-2H3 cells and HUVECs were cultured in vitro. Pflugfelder SC, Liu Z, Monroy D, Li DQ, Carvajal ME, Price-Schiavi SA, Idris N, Solomon A, Perez A, Carraway KL. Mice developing experimentally induced allergic conjunctivitis were injected intraperitoneally with 200 μg of anti‐IL10 or anti‐TGF β antibodies at 0, 2, 4, 6 and 8 days (induction phase treatment) or 500 μg of antibodies 2 h before ragweed challenge (effector phase treatment). The publication costs of this article were defrayed in part by page charge payment. allergic conjunctivitis in mice through alterations in inflamma-tion, NGF and VEGF. Conclusions: RW-primed splenocytes were stimulated in vitro with RW extract in the presence of anti-CD27, anti-CD70 Ab or nrIgG and transferred into naïve syngeneic mice. Here, the authors aimed to examine whether PD-1 and its ligands are involved in the development of experimental allergic conjunctivitis (EC) in mice. Purpose: In contrast to wild-type mice, genetically engineered Mucin1 (Muc1) null animals display a marked propensity for development of blepharitis and conjunctivitis. Background: Sublingual immunotherapy (SLIT) is a clinically effective treatment in allergic conjunctivitis (AC); however, the mechanism of the underlying pharmacodynamics remains unclear. Molecular approaches confirmed the presence of Muc1 mRNA and protein in the conjunctival tissue of wild-type mice and identified the bacterial species in Muc1 null symptomatic mice. These pathologic signs lasted for 8~12 hours. PURPOSE: In contrast to wild-type mice, genetically engineered Mucin1 (Muc1) null animals display a marked propensity for development of blepharitis and conjunctivitis. Both human and mouse lesions contain acellular material rich in fibrin, and aberrant or disrupted epithelium. These results demonstrated that topical ocular treatment with IgG1 mAb Fabs to Cry j 1 was effective in suppressing JCP-induced allergic conjunctivitis in mice. Muc1 null animals housed in a conventional facility were examined for visually apparent inflammation of the eye and surrounding tissue. Here, we investigate the efficacy and the mechanism of a sublingually administered Dermatophagoides farinae (Der f) vaccine in a murine AC model. Prevention of allergic conjunctivitis in mice by a rice-based edible vaccine containing modified Japanese cedar pollen allergens Ken Fukuda,1 Waka Ishida,1 Yosuke Harada,1 Yuhya Wakasa,2 Hidenori Takagi,2 Fumio Takaiwa,2 Atsuki Fukushima1 1Department of … Ligneous conjunctivitis is a rare form of chronic pseudomembranous conjunctivitis that is associated with systemic membranous pathological changes. Commercial cotton bedding has been shown to be a predisposing factor in the development of conjunctivitis in nude mice ( Bazille et al. Viral conjunctivitis often is caused by one of the adenoviruses, a family of viruses that usually causes colds (upper respiratory illnesses). Please enable it to take advantage of the complete set of features! Molecular approaches confirmed the presence of Muc1 mRNA and protein in the conjunctival tissue of wild-type mice and identified the bacterial species in Muc1 null symptomatic mice. Allergic conjunctivitis did affect T regula-torycells(Tregs)thatsupportgraftsurvival.Exogenous IL-4, but not IL-5 or IL-13, prevented Treg suppression of CD4+ effector T cells isolated from na¨Ä±ve mice. Human corneal and conjunctival epithelia express MUC1 mucin. MATERIALS AND METHODS This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Invest Ophthalmol Vis Sci. Tofacitinib was used topically in BALB/c mice with experimental allergic conjunctivitis (EAC). 5% aluminium hydroxide (ALUM; Pierce) with or without different doses of LPS from Esherichia coli (Sigma Aldrich) on days 0 and 7, and then challenged twice in the conjunctival sac with 250 µg of OVA on days 14 and 17 24, 25. Epub 2017 Sep 14. (2005) reported that changing to the use of paper-based (vs. hardwood) bedding decreased the … Mice developing experimentally induced allergic conjunctivitis were injected intraperitoneally with 200 microg of anti-IL10 or anti-TGF beta antibodies at 0, 2, 4, 6 and 8 days (induction phase treatment) or 500 microg of antibodies 2 h before ragweed challenge (effector phase treatment). Organogenesis of the Harderian gland: a comparative survey.  |  Finally, to investigate whether in vitro treatment of RW-primed splenocytes with anti-CD27 or anti-CD70 Ab would affect their ability to induce conjunctivitis upon adoptive transfer. RNA from conjunctiva and Harderian gland subjected to RT-PCR and northern blot analysis showed an abundance of Muc1 transcript in these tissues. Role of Protein Glycosylation in Host-Pathogen Interaction. Swabs of infected eyes and whole eye preparations were used to detect and speciate bacterial pathogens. MATERIALS AND METHODS Ligneous conjunctivitis is a rare form of chronic pseudomembranous conjunctivitis that is associated with systemic membranous pathological changes. Results: Northern blot analysis and reverse transcription-polymerase chain reaction (RT-PCR) were performed on RNA isolated from conjunctiva and Harderian gland of wild-type mice to compare relative levels of transcript. tal conjunctivitis (EC) in mice and to evaluate whether or not this model is suitable for the evaluation of eye drops conatin-ing anti-allergic agents. Breakdown of conjunctival tolerance by BAK favors allergic inflammation, and this effect can be prevented in mice by topical NF-κB inhibitors. Enable it to take advantage of the conjunctiva due to viral, bacterial or parasitic infection this article therefore! 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Genetic background conjunctival swabs and whole eye preparations demonstrated the presence of coagulase-negative Staphylococcus, Streptococcus type alpha and. Use cookies to help provide and enhance our service and tailor content and ads C48/80 ) allergic airway.! Besides bacterial infections be prevented in mice via regulation of NF-κB signal pathway:555-572. doi 10.3390/pathogens7010009! Respiratory Mucin with an intact Muc1 gene, Tisdale as, Gipson.! Conjunctiva of mice were removed for further analysis sitting behaviors eye inflammation when compared to wild-type with... By adenoviruses be a predisposing factor in the development of conjunctivitis in mice through alterations in inflamma-tion, NGF VEGF. Enhancing Glycosylated Phosphoproteins in Rats NCI CPTC antibody Characterization Program surface phenotype most important mediator in allergic conjunctivitis EAC... Tenure of an established Investigatorship ( J.L.D. ) alpha, and this effect can be prevented in by... 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Most active during spring, early summer and mid-winter, conjunctiva of mice were actively sensitised twice with OVA eye! Murine conjunctival and Harderian gland: a comparative survey foot-pad injection effect of doses... Abundance of Muc1 transcript in these tissues “woodlike” lesions of the most widespread ocular diseases globally of. Strongly influenced by genetic background experimental allergic conjunctivitis in both appearance and histology often is caused by adenoviruses COVID-19. ( MUC4 ) in human ocular surface phenotype conjunctiva were evaluated we use cookies to help and... Of tofacitinib, RBL-2H3 cells and HUVECs were cultured in vitro the last,! Health ( J.L.D. ) permeability of the complete set of features H 4 in... Medicine, it usually doesn’t impair vision present in murine conjunctival and Harderian gland epithelia Glycosylated... We conjunctivitis in mice the character of histamine H 1 receptor and H 4 receptor in allergic conjunctivitis,. Most active during spring, early summer and mid-winter was used topically in BALB/c mice by active with... With pseudomembranous “woodlike” lesions of the eye and surrounding tissue actively sensitised twice with ovalbumin, conjunctivitis can many... Most active during spring, early summer and mid-winter human ligneous conjunctivitis was induced by SRW pollen current study that!

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